Fig 3.  Vaccinia virion substructures.  (A) Negatively stained preparation of MV after mild treatment with NP40 and 2-mercaptoethanol, so that surface tubule elements remain intact (Wilton et al., 1995). Bar, 0.2 uM.  (B)  Isolated core in thin section.  Note lateral bodies still attached  (Ichihashi et al., 1984). (C) Isolated core visualized by cryo electron microscopy (Dubochet et al., 1994).  (D)  Isolated core visualized by negative staining (Wilton et al., 1995).

References:
Dubochet, J., Adrian, M., Richter, K., Garces, J., Wittek, R., (1994). Structure of intracellular mature vaccinia virus observed by cryoelectron microscopy. J.Virol. 68, 1935-1941.
Ichihashi, Y., Oie, M., Tsuruhara, T., (1984). Location of DNA-binding proteins and disulfide-linked proteins in vaccinia virus structural elements. J.Virol. 50, 929-938.
Wilton, S., Mohandas, A. R., Dales, S., (1995). Organization of the vaccinia envelope and relationship to the structure of intracellular mature virions. Virology 214, 503-511.